oxa 1 Search Results


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Proteintech anti oxa1l
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Santa Cruz Biotechnology pkc isoform selective
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Chem Impex International levofloxacin
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Lechnologies Research Inc 2-( n -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)2-deoxyglucose 2-nbdg
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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INCF bla ndm -carrying plasmids
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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AAT Bioquest 2-[n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-nbdg)
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Matreya LLC d-erythroc6-ceramide
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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FUJIFILM ammonium 7-fluoroben-2-oxa-1,3-diazo4-sulfonate (sbd-f
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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AnaSpec nbd-x (6-(n-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoic acid
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Synthon Chemicals GmbH s)-n-(1-aza-6-thiaspiro[2.5]oct-1-en-2-yl)proline methyl ester
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Setareh Biotech LLC iodoacetyl-7-nitrobenz-2-oxa-1,3-diazol ia–nbd amide
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Becton Dickinson n-(7-nitrobenz-2-oxa-1, 3-diazol-4yl)-phallacidin
FIG. 2. Transfected <t>PKC</t> isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC <t>isoform.</t> The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
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Image Search Results


FIG. 2. Transfected PKC isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC isoform. The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.

Journal: The Journal of biological chemistry

Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.

doi: 10.1074/jbc.275.3.1601

Figure Lengend Snippet: FIG. 2. Transfected PKC isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC isoform. The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.

Article Snippet: PKC isoform-selective (PKCa, sc-208; PKCd, sc-937; PKCh, sc-215; PKCe, sc-214) antibodies were from Santa Cruz Biotechnology.

Techniques: Transfection, Cell Culture, Expressing, Plasmid Preparation, Migration, Luciferase, Activity Assay

FIG. 3. Concentration dependence of PKC-dependent activa- tion of hINV gene expression. Cultured keratinocytes were trans- fected with 2 mg of pINV-241 and 0–2 mg of each PKC isoform. The total concentration of plasmid in each transfection was maintained constant at 4 mg by addition of empty expression vector. After 24 h extracts were prepared and assayed for luciferase activity.

Journal: The Journal of biological chemistry

Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.

doi: 10.1074/jbc.275.3.1601

Figure Lengend Snippet: FIG. 3. Concentration dependence of PKC-dependent activa- tion of hINV gene expression. Cultured keratinocytes were trans- fected with 2 mg of pINV-241 and 0–2 mg of each PKC isoform. The total concentration of plasmid in each transfection was maintained constant at 4 mg by addition of empty expression vector. After 24 h extracts were prepared and assayed for luciferase activity.

Article Snippet: PKC isoform-selective (PKCa, sc-208; PKCd, sc-937; PKCh, sc-215; PKCe, sc-214) antibodies were from Santa Cruz Biotechnology.

Techniques: Concentration Assay, Gene Expression, Cell Culture, Plasmid Preparation, Transfection, Expressing, Luciferase, Activity Assay

FIG. 8. Regulation of PKC isoform level by TPA. Keratinocytes were treated with 50 ng/ml TPA for 24 h followed by preparation of nuclear extracts. Equivalent quantities of extract, layered based on protein concentration, were electrophoresed on an 8% acrylamide gel and transferred to nitrocellulose for detection using PKCe-, -d-, and -h-specific antibodies. Binding of the primary antibody was detected by incubation with an appropriate secondary antibody, and binding was visualized using ECL technology.

Journal: The Journal of biological chemistry

Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.

doi: 10.1074/jbc.275.3.1601

Figure Lengend Snippet: FIG. 8. Regulation of PKC isoform level by TPA. Keratinocytes were treated with 50 ng/ml TPA for 24 h followed by preparation of nuclear extracts. Equivalent quantities of extract, layered based on protein concentration, were electrophoresed on an 8% acrylamide gel and transferred to nitrocellulose for detection using PKCe-, -d-, and -h-specific antibodies. Binding of the primary antibody was detected by incubation with an appropriate secondary antibody, and binding was visualized using ECL technology.

Article Snippet: PKC isoform-selective (PKCa, sc-208; PKCd, sc-937; PKCh, sc-215; PKCe, sc-214) antibodies were from Santa Cruz Biotechnology.

Techniques: Protein Concentration, Acrylamide Gel Assay, Binding Assay, Incubation