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Image Search Results
Journal: The Journal of biological chemistry
Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.
doi: 10.1074/jbc.275.3.1601
Figure Lengend Snippet: FIG. 2. Transfected PKC isoforms are expressed in keratino- cytes. A, cultured keratinocytes were transfected with expression plas- mids encoding indicated PKC isozymes (1) or empty expression vector (2). After 48 h, total cell extracts were prepared. Equal quantities of protein were electrophoresed, transferred to nitrocellulose, and immu- noblotted with PKC isozyme-specific antibodies. The arrows indicate migration of the each respective PKC isoform. The molecular masses are indicated in kilodaltons. B, keratinocytes were transfected with c-fos promoter reporter plasmid in the presence of expression plasmids encoding the conventional a, b1, and g PKC isozymes or empty expres- sion vector (EV). After 48 h, total cell extracts were prepared and assayed for luciferase activity.
Article Snippet:
Techniques: Transfection, Cell Culture, Expressing, Plasmid Preparation, Migration, Luciferase, Activity Assay
Journal: The Journal of biological chemistry
Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.
doi: 10.1074/jbc.275.3.1601
Figure Lengend Snippet: FIG. 3. Concentration dependence of PKC-dependent activa- tion of hINV gene expression. Cultured keratinocytes were trans- fected with 2 mg of pINV-241 and 0–2 mg of each PKC isoform. The total concentration of plasmid in each transfection was maintained constant at 4 mg by addition of empty expression vector. After 24 h extracts were prepared and assayed for luciferase activity.
Article Snippet:
Techniques: Concentration Assay, Gene Expression, Cell Culture, Plasmid Preparation, Transfection, Expressing, Luciferase, Activity Assay
Journal: The Journal of biological chemistry
Article Title: Regulation of human involucrin promoter activity by novel protein kinase C isoforms.
doi: 10.1074/jbc.275.3.1601
Figure Lengend Snippet: FIG. 8. Regulation of PKC isoform level by TPA. Keratinocytes were treated with 50 ng/ml TPA for 24 h followed by preparation of nuclear extracts. Equivalent quantities of extract, layered based on protein concentration, were electrophoresed on an 8% acrylamide gel and transferred to nitrocellulose for detection using PKCe-, -d-, and -h-specific antibodies. Binding of the primary antibody was detected by incubation with an appropriate secondary antibody, and binding was visualized using ECL technology.
Article Snippet:
Techniques: Protein Concentration, Acrylamide Gel Assay, Binding Assay, Incubation